Tabriz University of Medical Sciences About    Newsletter    Contact Us    Create Account    Log in  

ISSN: 1735-403X      eISSN: 2383-2886  
Services
Export citation
EndNote
Reference Manager
BibTeX
Medlars
Refworks
Mendeley

Cite by
Google Scholar



Article History
Submitted: 09 Jan 2017
Accepted: 11 May 2017

Article Access Statistics
Abstract Page Views: 169
PDF Downloads: 308
Full Text Views: 0

Pharm Sci. 2017;23(2):150-158 doi: 10.15171/PS.2017.22

Halorubrum sp. TBZ112, an Extremely Halophilic Carotenoid- Producing Archaeon Isolated from Urmia Lake

Research Article

Masoud Hamidi 1,2 * , Mohammad Saeid Hejazi 2, Hossein Nazemyieh 3, Mohammad Amin Hejazi 4, Davood Naziri 5

1 Department of Medical Biotechnology, School of Paramedicine, Guilan University of Medical Sciences, Rasht, Iran.
2 Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.
3 Department of Pharmacognosy, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.
4 West & Northwest Agricultural Biotechnology Research Institute of Iran (ABRII), Tabriz, Iran.
5 Agricultural Biotechnology Research Institute of Iran (ABRII), Karaj, Iran.



Abstract

Background: Carotenoids are organic pigments with substantial applications in neutraceuticals, pharmaceuticals, cosmetics and food industries. Considering the importance of carotenoids, we aimed to isolate and identify a carotenoid-producing microorganism in the present study.

Methods: Gram-negative, aerobic and rod-shaped archaeon, Halorubrum sp. TBZ112 (KCTC 4203 and IBRC-M 10773) producing carotenoids with circle and red colonies were isolated from Urmia Lake in Northwest Iran. Phenotypic characterization and molecular identification of isolate were also conducted. In order to study the ability of TBZ112 to produce carotenoids as well as the production profile, carotenoids were extracted using acetone-methanol solution (7:3 v/v). Then, the carotenoid content of the extract was evaluated using UV spectroscopy and thin-layer chromatography (TLC). The carotenoid profile was analyzed using liquid chromatography-mass spectrometry (LC-MS) techniques.

Results: The 16S rRNA analysis showed that TBZ112 had the highest similarity with Halorubrum chaoviator Halo-G*T (99.78%). The total carotenoid content of strain TBZ112 was found to be 11.7 mg/l. The LC-MS analytical results indicated that carotenoids that were produced included bacterioruberin, lycopene and β-carotene. Among them, bacterioruberin was predominant.

Conclusion: Consequently, we can suggest that future studies should investigate this new and natural source for producing carotenoids.







Comments
First name  
Last name  
Email address  
Comments  
Security code



This Article
PDF

Google Scholar
Articles by Hamidi M
Articles by Hejazi MS
Articles by Nazemyieh H
Articles by Hejazi MA
Articles by Naziri D

PubMed
Articles by Hamidi M
Articles by Hejazi MS
Articles by Nazemyieh H
Articles by Hejazi MA
Articles by Naziri D



Share this article!
© 2016 Tabriz University of Medical Sciences; unless otherwise stated
Press Manuscript Online. Powered by MAADRAYAN