Tabriz University of Medical Sciences About    Newsletter    Contact Us    Create Account    Log in  

ISSN: 1735-403X      eISSN: 2383-2886  
Services
Export citation
EndNote
Reference Manager
BibTeX
Medlars
Refworks
Mendeley

Cite by
Google Scholar



Article History
Submitted: 25 Nov 2015
Accepted: 13 Dec 2015
First published online: 30 Mar 2016

Article Access Statistics
Abstract Page Views: 528
PDF Downloads: 869
Full Text Views: 0

Pharm Sci. 2016;22(1):28-34 doi: 10.15171/PS.2016.06

Determination of Some Cephalosporins in Pharmaceutical Formulations by a Simple and Sensitive Spectrofluorimetric Method

Research Article

Ali Abdollahi 1, Ahad Bavili-Tabrizi 2 *

1 Drug Applied Research Center and Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran
2 Biotechnology Research Center and Faculty of Pharmacy , Tabriz University of Medical Sciences, Tabriz, Iran



Abstract
Background: Cephalosporins are among the safest and the most effective broad-spectrum bactericidal antimicrobial agents which have been prescribed by the clinician as antibiotics. Thus, the developing of simple, sensitive and rapid analytical methods for their determination can be attractive and desirable. Methods: A simple, rapid and sensitive spectrofluorimetric method was developed for the determination of cefixime, cefalexin and ceftriaxone in pharmaceutical formulations. Proposed method is based on the oxidation of these cephalosporins with cerium (IV) to produce cerium (III), and its fluorescence was monitored at 356 ± 3 nm after excitation at 254 ± 3 nm. Results: The variables effecting oxidation of each cephalosporin with cerum (IV) were studied and optimized. Under the experimental conditions used, the calibration graphs were linear over the range 0.1-4 µg/mL. The limit of detection and limit of quantification were in the range 0.031-0.054 and 0.102-0.172 µg/mL, respectively. Intra- and inter-day assay precisions, expressed as the relative standard deviation (RSD), were lower than 5.6 and 6.8%, respectively. Conclusion: The proposed method was applied to the determination of studied cephalosporins in pharmaceutical formulations by good recoveries in the range 91-110%.





Comments
First name  
Last name  
Email address  
Comments  
Security code



This Article
PDF

Google Scholar
Articles by Abdollahi A
Articles by Bavili-Tabrizi A

PubMed
Articles by Abdollahi A
Articles by Bavili-Tabrizi A



Share this article!
© 2016 Tabriz University of Medical Sciences; unless otherwise stated
Press Manuscript Online. Powered by MAADRAYAN