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Article History
Submitted: 07 Jun 2017
Revised: 20 Nov 2017
Accepted: 23 Nov 2017
First published online: 15 Mar 2018

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Pharm Sci. 2018;24(1):15-22 doi: 10.15171/PS.2018.04

Fatty Acid Mixtures from Nigella sativa Protects PC12 Cells from Oxidative Stress and Apoptosis Induced by Doxorubicin

Research Article

Leila Hosseinzadeh 1, Shima Soheili 2, Nastaran Ghiasvand 1, Farahnaz Ahmadi 1, Yalda shokoohinia 1,3 *

1 Pharmaceutical Sciences Research Center, School of Pharmacy, Kermanshah University of Medical Sciences, Kermanshah, Iran.
2 Student Research Committee, School of Pharmacy, Kermanshah University of Medical Sciences, Kermanshah, Iran.
3 Department of Pharmacognosy and Biotechnology, School of Pharmacy, Kermanshah University of Medical Sciences, Kermanshah, Iran.



Abstract
Background: Fatty acids (FAs), the key structural elements of dietary lipids, are notable in the nutritional value of plants. Black cumin, a popular anti-inflammatory and antioxidant food seasoning, contains nonpolar constituents such as FAs. Methods: Seeds were extracted using hexane and their cytoprotective activity was assessed against doxorubicin (DOX)-mediated oxidative stress and apoptosis in PC12 cell line. Results: In spite of the cellular death induced by DOX toward PC12 cells, bioassay-guided purification showed that pretreatment with FAs mixtures (24h) attenuated DOX-mediated apoptosis, which could be attributed to the inhibited caspase 3 activity and enhanced mitochondrial membrane potential. Palmitic acid, caprylic acid and oleic acid each 1/3 in the mixture, also suppressed DOX-induced ROS generation. Conclusion: Our observation indicated that the subtoxic concentration of FAs from Nigella sativa could effectively protect the cells against oxidative stress, due to their antioxidant activity, and could be regarded as a dietary supplement.





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