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Article History
Submitted: 02 Aug 2017
Revised: 17 Mar 2018
Accepted: 18 Mar 2018
First published online: 20 Jun 2018

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Pharm Sci. 2018;24(2):124-130 doi: 10.15171/PS.2018.19

Effect of Platelet-Rich Plasma on Differentiation of Osteoblasts and Osteoclasts in the Presence of Three-Dimensional Scaffold

Research Article

Hadi Mokhtari 1, Azadeh Montaseri 2, Ali Mojaddadi 1 * , Hamid Reza Mokhtari Zonouzi 1, Nahid Karimiyan 2, Saba Arami 3

1 Department of Endodontics, Faculty of Dentistry, Tabriz University of Medical Sciences, Tabriz, Iran.
2 Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
3 Department of Aesthetic and Operative, Faculty of Dentistry, Tabriz University of Medical Sciences, Tabriz, Iran.



Abstract
Background: Osteoblasts’ activity is prerequisite for prevention from and treatment of apical periodontitis and a relatively high proportion of endodontically treated teeth will require retrograde treatment in future. Therefore, the aim of the present study was to evaluate the effect of platelet-rich plasma (PRP) on differentiation of stem cells into osteoblasts and osteoclasts. Methods: Mesenchymal stem cells were isolated from human fetal umbilical cord and cultured on two polycaprolacton/hydroxyapatite (PCL/HA) polymer scaffolds. In addition to differentiation agents, 10% PRP was added to PRP containing subgroups. After 10 days, osteoblast differentiation was assessed evaluating the osteocalcin and osterix gene levels where, in the osteoclast differentiation group the expression of tartarate-resistant acid phosphatase (TRAP) gene was evaluated. Results: Expression of TRAP gene did not reveal any significant differences between the study and control groups. There was a significant difference in osterix expression between the control and the PRP-treated groups (p < 0.01) as well as osteocalcin gene (p < 0.05). Conclusion: The results showed that PRP increased the osteoblastic differentiation, while it does not cause any significant increase in osteoclastic differentiation.





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