Pharm Sci. 2016;22(4):227-233 doi: 10.15171/PS.2016.36
of Sodium Selenite on Formaldehyde Induced Renal Toxicity in Mice
Background: Formaldehyde is widely used for industrial
applications. Renal injury is an adverse effect associated with formaldehyde.
Few studies have explored the potential benefits of protective factors on
formaldehyde induced renal toxicity. This study evaluated the dose dependent
effects of sodium selenite on the biochemical and histopathological effects of
formaldehyde on murine kidney.
Methods: Forty eight adult Balb/c male mice were randomized into six groups: a
control group, a formaldehyde group and experimental III-VI groups. Formaldehyde
group was injected with 10 mg/kg formaldehyde and groups III-VI received
intraperitoneally doses of 0.1, 0.2, 0.4, 0.8 mg/kg selenium. After two weeks,
a stereological study was done in accordance with the principle of Cavalieri
and serum concentrations of urea and creatinine were measured. Data were
analyzed using ANOVA and SPSS software.
Results: Glomerosclerosis, necrosis and vacuolization were observed in the
convoluted tubules of animals treated with formaldehyde. The biochemical
markers, volume and count of glomeruli in the group treated with formaldehyde
was significantly difference compared to the control group (P<0.05). The
volume of the glomeruli in the group treated with 0.2 and 0.4 mg selenium and
urea level in the group treated with 0.4 and 0.1 mg/kg selenium was
significantly difference compared to the control group (P <0.05). The count
of glomeruli and creatinine level in the selenium group was significantly
difference compared to the control group (P ≤ 0.0001).
Conclusions: A dose of 0.2 mg/kg of sodium
selenite caused partial protective effect on the renal tissue and function in
exposed to formaldehyde.