Mojtaba Kalantar
1,2, Saeed Shirali
3, Amin Hasanvand
4, Masoud Valizadeh
5, Ramin Tavakoli
3, Marzieh Asadi
6, Mehdi Goudarzi
2,4*1 Shoushtar Faculty of Medical Sciences, Shoushtar, Iran
2 Student Research Committee, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
3 Research Center of Thalassemia and Hemoglobinopathy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
4 Department of Pharmacology and Toxicology, Faculty of Pharmacy, Lorestan University of Medical Sciences, Khorramabad, Iran
5 Department of Toxicology, School of Pharmacy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
6 Department of Biology, Faculty of Science, Shahid Chamran University, Ahvaz, Iran
Abstract
Background: Methotrexate
as a chemotherapy drug can causes chronic liver damage and oxidative stress.
The aim of this study was to evaluate the protective effect of hydroalcoholic
extract of Lavandula officinalis on methotrexate-induced oxidative
stress in rats.
Methods: In this
experimental study, thirty five Wistar male rats weighting 200-250 g were
randomly divided into 5 groups (n = 7 in each group). Negative control group
(normal saline 5ml/kg); positive control group received normal salin orally for
10 days, and a single dose of methotrexate (MTX, 20mg/kg, i.p.) was administrated on the 9th day. Groups 3-5
received respectively 100, 200 and 400 mg/kg of Lavandula officinalis
extract (LOE) orally for 10 days, and a single dose of MTX was injected on the
9th day. 24 h after the last injection, animals
were sacrificed. Blood samples were collected to determine serum AST, ALT
and ALP levels. Malondialdehyde
(MDA), glutathione (GSH) levels and catalase (CAT), superoxide dismutase (SOD)
and glutathione peroxidase (GPx) activity were assayed in liver tissue. A
portion of liver was maintained in 10% formalin for Hematoxylin and Eosin
(H&E) staining and histological examination.
Results: The result obtained from current study was showed a
significant increase in the levels of AST, ALT, ALP, MDA and decrease of GSH, CAT and SOD by MTX administration.
Pre-treatment with LOE showed reduction in the levels of AST, ALT, ALP, MDA and increase of GSH, CAT and SOD in all doses but
the most significant alteration was observed in doses of 200 and 400 mg/kg
(P<0.05). Histological results showed that methotrexate could lead to liver damage. Also the hepatoprotective effect of the LOE was
confirmed by the histological examination of the liver.
Conclusion: Our results indicate
that hydroalcoholic
extract of Lavandula officinalis have produced amelioration in
biochemical and oxidative stress parameters against MTX -induced oxidative
stress.