Pharm Sci. 2017;23(1):72-76 doi: 10.15171/PS.2017.11
Variations in Antibiotic Susceptibility Profile of Staphylococcus aureus after Povidone-Iodine
one of the major causes of nosocomial infection which is the most commonly
identified agent responsible for skin and soft tissue infections. Cleaning of
the skin before an intervention remarkably reduces the risk of infections. The
aim of this study was to investigate the effects of povidone-iodine stress on
the antimicrobial susceptibility pattern of S. aureus resistant
Methods: A 24 h fresh culture was prepared by S.
aureus inoculation into the tube containing trypticase soy broth (TSB).
Then an povidone–iodine resistant subpopulation of strain was selected by
exposure to povidone-iodine stress with concentrations 3% and 10%
(wt/vol). The resulting survived cells were maintained in 10 mL TSB. The
Antibiotic susceptibility testing was performed on strains by disk diffusion
method according to CLSI recommendations.
Results: Evaluation of antimicrobial susceptibility pattern
revealed significant differences in zone of inhibitions between stressed and
unstressed strains. S. aureus strains stressed at 3% povidone-iodine
concentrations showed statistically smaller zone of inhibition against
clindamycin, methicillin and chloramphenicol compared to unstressed strains.
Although, 3% povidone-iodine stressed strains were become more susceptible to penicillin
G and cephalothin.
Rifampicin, ciprofloxacin, methicillin and
chloramphenicol in stressed strains showed a significant decrease in zone of
inhibition at 10% povidone-iodine concentrations, and higher sensitivity to
co-trimoxazole, cephalothin and cephalexin.
observed changes in antimicrobial susceptibility patterns of S. aureus
strains can contribute to povidone–iodine stresses. Although extend study on
wider range of skin and wound associated pathogens necessitate to reach a comprehensive