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Article History
Submitted: 12 Jan 2017
Revised: 02 May 2017
Accepted: 02 May 2017
First published online: 15 Mar 2018

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Pharm Sci. 2018;24(1):71-78 doi: 10.15171/PS.2018.11

Microextraction of Furosemide from Human Serum and Its Fluorimetric Determination

Research Article

Akbar Rezazadeh 1,2, Mohammad Amjadi 3, Jamshid L Manzoori 4, Alireza Ghaffari 5, Abolghasem Jouyban 1,6 *

1 Pharmaceutical Analysis Research Center and Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.
2 Research & Development, Shahid Ghazi Pharmaceutical Company, Tabriz, Iran.
3 Department of Analytical Chemistry, Faculty of Chemistry, University of Tabriz, Tabriz, Iran.
4 Department of Chemistry, Tabriz Branch, Islamic Azad University, Tabriz, Iran.
5 Medical Philosophy and History Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
6 Kimia Idea Pardaz Azarbayjan (KIPA) Science Based Company, Tabriz University of Medical Sciences, Tabriz, Iran.



Abstract
Background: A new, fast and sensitive spectrofluorimetric method was proposed for the determination of furosemide in serum samples based on a dispersive liquid-liquid microextraction. Methods: The optimum conditions for quantification of furosemide were studied considering the effects of types and amounts of dispersive and extraction solvents, salt addition, pH value, rate and duration of centrifugation. The method was validated with respect to the linearity, recovery and limit of detection. Results: Under the optimal conditions, the fluorescence intensities at 406 nm (with the excitation wavelength of 342 nm) were linear with the concentration of furosemide in the range of 0.3 to 20 µg mL-1, with a detection limit of 0.12 µg mL-1 and a relative standard deviation of 3.4–9.4%. Conclusion: Careful examination of the obtained validation results reveal that the proposed method is suitable for determination of furosemide in serum samples.





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Articles by Rezazadeh A
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Articles by Amjadi M
Articles by L Manzoori J
Articles by Ghaffari A
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