Tabriz University of Medical Sciences About    Newsletter    Contact Us    Create Account    Log in  
Journal of Analytical Research in Clinical Medicine
   eISSN: 2345-4970  
Services
Export citation
EndNote
Reference Manager
BibTeX
Medlars
Refworks
Mendeley

Cite by
Google Scholar



Article History
Submitted: 30 Nov 2016
Revised: 27 Dec 2016
Accepted: 02 Jan 2017
First published online: 10 Mar 2017

Article Access Statistics
Abstract Page Views: 12
PDF Downloads: 20
Full Text Views: 0

J Anal Res Clin Med. 2017;5(1):15-19 doi: 10.15171/jarcm.2017.004

Sex determination using free fetal DNA in early pregnancy: With the approach to sex linked recessive disorders

Original Article

Amir Monfaredan 1, Shahrokh Amiri, Seyed Mahmoud Tabatabaei *

1 Department of Lab Sciences, School of Medicine, Tabriz Branch, Islamic Azad University, Tabriz, Iran
2 Research Center of Psychiatry and Behavioral Sciences, Tabriz University of Medical Sciences, Tabriz, Iran
3 Department of Physiology, School of Medicine, Tabriz Branch, Islamic Azad University, Tabriz, Iran



Abstract
Introduction: Prenatal diagnosis is testing for detection of diseases or conditions in a fetus or embryo before it is born. Most of prenatal diagnostic (PD) techniques are invasive and done in late stages of pregnancy. Using fetal DNA in maternal blood for fetal sex determination in early pregnancy might help in management of X-linked genetic diseases. This study aimed to investigate the accuracy of sex determination using fetal DNA in maternal blood at 8-12 weeks of gestation. Methods: In this cross-sectional study, 30 pregnant women at 8-12 weeks of gestation were enrolled. The sex-determining region Y (SRY) gene expression with the internal control (IC) glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was investigated with quantitative real-time polymerase chain reaction (PCR) using specific primers and probes. Results: Accuracy of sex determination with SRY gene expression in 8-12 weeks of pregnancy were 85%, 85%, 90% and 100% respectively. Conclusion: It seems that fetal sex determining using fetal DNA in maternal blood is a reliable method for early stage of pregnancy.





Comments
First name  
Last name  
Email address  
Comments  
Security code



This Article
PDF

Google Scholar
Articles by Monfaredan A
Articles by Amiri S
Articles by Tabatabaei SM

PubMed
Articles by Monfaredan A
Articles by Amiri S
Articles by Tabatabaei SM



Share this article!
© 2017 Tabriz University of Medical Sciences; unless otherwise stated.

Press Manuscript Online. Powered by MAADRAYAN