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Advanced Pharmaceutical Bulletin
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Article History
Submitted: 22 Aug 2013
Revised: 28 Aug 2013
First published online: 23 Dec 2013

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Adv Pharm Bull. 2014;4(1):49-54 doi: 10.5681/apb.2014.008
PMID:24409409        PMCID:PMC3885368

The Anti-Inflammatory Effect of Erythropoietin and Melatonin on Renal Ischemia Reperfusion Injury in Male Rats

Original Research

Nasser Ahmadiasl 1, Shokofeh Banaei 2 * , Alireza Alihemmati 3, Behzad Baradaran 4, Ehsan Azimian 5

1 Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
2 Department of Physiology, Tabriz University of Medical Sciences, Tabriz, Iran.
3 Department of Histology & Embryology, Tabriz University of Medical Sciences, Tabriz, Iran.
4 Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
5 Department of Linguistics and Foreign Languages, Payame Noor University, Tehran, Iran.



Abstract
Purpose: Renal ischemia reperfusion (IR) is an important cause of renal dysfunction. It contributes to the development of acute renal failure (ARF). The purpose of this study was to investigate the anti-inflammatory effect of erythropoietin (EPO) and melatonin (MEL), which are known anti-inflammatory and antioxidant agents, in IR-induced renal injury in rats. Methods: Male Wistar Albino rats were unilaterally nephrectomized and subjected to 45 min of renal pedicle occlusion followed by 24 h reperfusion. MEL (10mg/kg, i.p) and EPO (5000U/kg, i.p) were administered prior to ischemia. After 24 h reperfusion, blood samples were collected for the determination of total antioxidant capacity (TAC), malondialdehyde (MDA) and serum creatinine levels. Also, renal samples were taken for Immunohistochemical evaluation of Bcl2 and TNF-α (tumor necrosis factor-α) expression. Results: Ischemia reperfusion increased creatinine, TAC, MDA levels and TNF-α expression, also, IR decreased Bcl2 expression. Treatment with EPO or MEL decreased creatinine, MDA levels, and increased TAC level. Also, MEL up-regulated Bcl2 expression and down-regulated TNF-α expression compared with EPO. Conclusion: Treatment with EPO and MEL had a curative effect on renal IR injury. These results may indicate that MEL protects against inflammation and apoptosis better than EPO in renal IR injury.





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