Adv Pharm Bull. 2016;6(4):645-650 doi: 10.15171/apb.2016.079
The Effects of Hypoxia on U937 Cell Line in Mesenchymal Stem Cells
Purpose: Mesenchymal Stem Cells (MSCs) are the most important
members of Bone Marrow (BM) milieu. MSCs affect different kinds of cells,
particularly malignant cells of hematologic malignancies, but the effects of
MSCs are unclear exactly. Here we analyzed the effects of derived Umbilical
Cord Blood-MSCs on proliferation, cell death and some surface markers of U937
cell line in a Co-culture system with MSCs.
Methods: Here we designed Co-culture systems as a model of BM
milieu. We cultured U937 cells on UCB-MSCs and MSCs Conditioned Medium (C.M)
driven and then treated U937 cells with optimum concentration of chloride
cobalt (CoCl2) as a hypoxia-mimetic agent. In addition, we applied
suitable concentrations of H2O2 to induce cell death.
Proliferation rate, cell death rate and some surface markers of hypoxic U937
cells were analyzed by MTT assay, flow cytometry and Real Time-PCR were flown
Results: UCB-MSCs showed supportive effects on U937
proliferation rate in normoxia and hypoxia. Lethal effect of H2O2
suppressed in the presence of UCB-MSCs in hypoxia and normoxia. Among
CD11a, CD14, CD49d, CD54 and CD116 markers, CD49d was down regulated in
presence of UCB-MSCs and CD116 was up regulated in hypoxia. Other markers
didn’t show any significant changes.
Conclusion: This work provides evidences that MSCs play critical
roles in U937 cells biology. These observations shed new light on MSCs roles
and demonstrated that MSCs should be regarded as an important member of BM
milieu in several clinical applications such as BM transplantation prognosis
and treatment of hematologic malignancies.