Tabriz University of Medical Sciences About    Newsletter    Contact Us    Create Account    Log in  
Advanced Pharmaceutical Bulletin
ISSN: 2228-5881      eISSN: 2251-7308  
Services
Export citation
EndNote
Reference Manager
BibTeX
Medlars
Refworks
Mendeley

Cite by
Google Scholar
PMC(3)


Article History
Submitted: 09 Sep 2013
Revised: 26 Sep 2013
First published online: 23 Dec 2013

Article Access Statistics
Abstract Page Views: 244
PDF Downloads: 133
Full Text Views: 0

Adv Pharm Bull. 2014;4(1):61-67 doi: 10.5681/apb.2014.010
PMID:24409411        PMCID:PMC3885371

Analysis of Carotenoid Production by Halorubrum sp. TBZ126; an Extremely Halophilic Archeon from Urmia Lake

Original Research

Davood Naziri 1,2, Masoud Hamidi 1,3, Salar Hassanzadeh 1, Vahideh Tarhriz 1, Bahram Maleki Zanjani 2, Hossein Nazemyieh 4, Mohamm Amin Hejazi 5, Mohammad Saeid Hejazi 1 *

1 Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.
2 Department of Agricultural Biotechnology, Faculty of Agriculture, Zanjan University, Zanjan, Iran.
3 Students’ Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran.
4 Department of Pharmacognosy, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.
5 Branch for Northwest & West Region, Agricultural Biotechnology Research Institute of Iran (ABRII), Tabriz, Iran.



Abstract
Purpose: Carotenoids are of great interest in many scientific disciplines because of their wide distribution, diverse functions and interesting properties. The present report describes a new natural source for carotenoid production. Methods: Halorubrum sp., TBZ126, an extremely halophilic archaeon, was isolated from Urmia Lack following culture of water sample on marine agar medium and incubation at 30 °C. Then single colonies were cultivated in broth media. After that the cells were collected and carotenoids were extracted with acetone-methanol (7:3 v/v). The identification of carotenoids was performed by UV-VIS spectroscopy and confirmed by thin layer chromatography (TLC) in the presence of antimony pentachloride (SbCl5). The production profile was analyzed using liquid-chromatography mass spectroscopy (LC-MS) techniques. Phenotypic characteristics of the isolate were carried out and the 16S rRNA gene was amplified using polymerase chain reaction (PCR). Results: LC-MS analytical results revealed that produced carotenoids are bacterioruberin, lycopene and β-carotene. Bacterioruberin was found to be the predominant produced carotenoid. 16S rRNA analysis showed that TBZ126 has 100% similarity with Halorubrum chaoviator Halo-G*T (AM048786). Conclusion: Halorubrum sp. TBZ126, isolated from Urmia Lake has high capacity in the production of carotenoids. This extremely halophilic archaeon could be considered as a prokaryotic candidate for carotenoid production source for future studies.





Comments
First name  
Last name  
Email address  
Comments  
Security code



This Article
PDF

Google Scholar
Articles by Naziri D
Articles by Hamidi M
Articles by Hassanzadeh S
Articles by Tarhriz V
Articles by Maleki Zanjani B
Articles by Nazemyieh H
Articles by Hejazi MA
Articles by Hejazi MS

PubMed
Articles by Naziri D
Articles by Hamidi M
Articles by Hassanzadeh S
Articles by Tarhriz V
Articles by Maleki Zanjani B
Articles by Nazemyieh H
Articles by Hejazi MA
Articles by Hejazi MS

Similar articles in PubMed

Share this article!

Press Manuscript Online. Powered by MAADRAYAN