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Advanced Pharmaceutical Bulletin
ISSN: 2228-5881      eISSN: 2251-7308  
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Article History
Submitted: 23 Feb 2013
Revised: 15 May 2013
First published online: 20 Aug 2013

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Adv Pharm Bull. 2013;3(2):473-476 doi: 10.5681/apb.2013.079
PMID:24312882        PMCID:PMC3848235

Construction of Yeast Recombinant Expression Vector Containing Human Epidermal Growth Factor (hEGF)

Short Communication

Jamal Mohammadian, Sima Mansoori-Derakhshan, Masood Mohammadian, Mahmoud Shekari-Khaniani *


Abstract
Purpose: The objective of this study was construction of recombinant hEGF-pPIC9 which may be used for expression of recombinant hEGF in following studies. Methods: EGF cDNA was purchased from Genecopoeia Company and used for PCR amplification. Prior to ligation, the PCR product and pPIC9 vector was digested with EcoRI and XhoI and ligated in pPIC9 vector and subjected to colony PCR screening and sequencing analysis. Results: PCR amplification of EGF cDNA using recombinant hEGF-pPIC9 vector as template was concluded in amplification of 197bp fragment. Construction of recombinant hEGF-pPIC9 of EGf gene was verified by PCR and sequencing. Conclusion: Construction of Recombinant hEGF-pPIC9 was the primary stage for production and expression of EFG in the future study.





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