Adv Pharm Bull. 2017;7(1):87-95 doi: 10.15171/apb.2017.011
In vitro Cytotoxicity Effects
of 197Au/PAMAMG4 and 198Au/PAMAMG4 Nanocomposites Against
MCF7 and 4T1 Breast Cancer Cell Lines
on gold based therapeutic agents for cancer cells deracination has become under
scrutiny in recent years owing to effective treatments are not available for
rapidly progressive cancers. The aim of present study was to examine efficiency
of radioactive 198Au/PAMAMG4 and non-radioactive 197Au/PAMAMG4
nancomposites against 4T1 and MCF7 breast cancer cell lines.
Methods: The PAMAMG4 dendrimer was treated with the
gold anions and then, the mixture was chemically reduced by NaBH4.
Prepared 197Au/PAMAMG4 was bombarded by thermal neutrons in the
Tehran Research Reactor to 198Au/PAMAMG4 be produced. Prepared
nanocomposites were characterized by means of FT-IR, 1H NMR, Zeta-potential
measurements, TEM and EDX analyses. The radionuclidic purity of the 198Au/PAMAMG4
solution was determined using purity germanium (HPGe) spectroscopy and its
stability in the presence of human serum was studied. In vitro studies were
carried out to compare toxicity of PAMAMG4, 197Au/PAMAMG4 and 198Au/PAMAMG4
towards 4T1 and MCF7 cancerous cells and C2C12 normal cell lines.
Results: Characterization results exhibited that
invitro agents, 197Au/PAMAMG4 and 198Au/PAMAMG4, were
synthesized successfully. Cells viability after 24 h, 48 h, and 72 h
incubation, using MTT assay showed that the toxicity of 198Au/PAMAMG4
is significantly superior in comparison with 197Au/PAMAMG4 and
PAMAMG4. Furthermore, the toxicity of 198Au/PAMAMG4 was higher on
cancerous cells especially in higher level of concentrations after 72 hours
Conclusion: In the current study, the preparation of 197Au/PAMAMG4
and 198Au/PAMAMG4 is described and the cytotoxic properties of them
against the MCF7, 4T1 cancerous cells and C2C12 normal cells were evaluated
using MTT assay.