Tabriz University of Medical Sciences About    Newsletter    Contact Us    Create Account    Log in  
Advanced Pharmaceutical Bulletin
ISSN: 2228-5881      eISSN: 2251-7308  
Services
Export citation
EndNote
Reference Manager
BibTeX
Medlars
Refworks
Mendeley

Cite by
Google Scholar
PMC(1)


Article History
Submitted: 14 Feb 2013
Revised: 07 Apr 2013
First published online: 20 Aug 2013

Article Access Statistics
Abstract Page Views: 157
PDF Downloads: 113
Full Text Views: 0

Adv Pharm Bull. 2013;3(2):461-464 doi: 10.5681/apb.2013.076
PMID:24312879        PMCID:PMC3848230

Evaluation of STAT5A Gene Expression in Aflatoxin B1 Treated Bovine Mammary Epithelial Cells

Short Communication

Ali Forouharmehr * , Taher Harkinezhad * , Babak Qasemi-Panahi


Abstract
Purpose: Aflatoxin B1 (AFB1) is a potent mycotoxin which has been produced by fungi such as Aspergillus flavus and Aspergillus parasiticus as secondary metabolites due to their growth on food stuffs and induces hepatocellular carcinoma in many animal species, including humans. In the present study, the effect of AFB1 on STAT5A gene expression was investigated in bovine mammary epithelial cells using real time RT-PCR. Methods: Bovine mammary epithelial cells were seeded in a 24-well culture plate for three-dimensional (3D) culture in Matrigel matrix. After 21 days of 3D culture and reaching the required number of cells, cells were treated with AFB1 and incubated for 8 h. For real time PCR reaction, total RNA from the cultured and treated cells was extracted and used for complementary DNA synthesis. Results: The expression of STAT5A gene was significantly down regulated by AFB1 in dose- dependent manner and led to the reduction of proliferation and differentiation of epithelial cells, which has direct effect in milk protein quantity and quality. Conclusion: According to the results, it seems that down regulation of STAT5A gene in AFB1-treated cells maybe due to DNA damage induced by AFB1 in bovine mammary epithelial cells.





Comments
First name  
Last name  
Email address  
Comments  
Security code



This Article
PDF

Google Scholar
Articles by Forouharmehr A
Articles by Harkinezhad T
Articles by Qasemi-Panahi B

PubMed
Articles by Forouharmehr A
Articles by Harkinezhad T
Articles by Qasemi-Panahi B

Similar articles in PubMed

Share this article!

Press Manuscript Online. Powered by MAADRAYAN