Tabriz University of Medical Sciences About    Newsletter    Contact Us    Create Account    Log in  
Advanced Pharmaceutical Bulletin
ISSN: 2228-5881      eISSN: 2251-7308  
Services
Export citation
EndNote
Reference Manager
BibTeX
Medlars
Refworks
Mendeley

Cite by
Google Scholar
PMC(1)


Article History
Submitted: 04 Dec 2012
Revised: 29 Dec 2012
First published online: 07 Feb 2013

Article Access Statistics
Abstract Page Views: 198
PDF Downloads: 87
Full Text Views: 0

Adv Pharm Bull. 2013;3(1):231-237 doi: 10.5681/apb.2013.038
PMID:24312841        PMCID:PMC3846032

Extractive Spectrophotometric Determination of Ambrisentan

Original Research

Namasani Santhosh Kumar, Avula Prameela Rani, Telu Visalakshi, Chandra Bala Sekharan *


Abstract
Purpose: Ambrisentan (ABS) is an antihypertensive drug used in the treatment of pulmonary atrial hypertension. The survey of literature for ABS revealed only two spectrophotometric methods for its quantification. The reported methods lack the sensitivity. This study is aimed at developing two sensitive extractive spectrophotometric methods for the determination of ABS in bulk and in tablets. Methods: The proposed methods are based on the formation of colored chloroform extractable ion-pair complexes of ABS with methylene blue (MB method) and safranine O (SO method) in buffered solution at pH 9.8. The extracted complexes showed maximum absorbance at 525 and 515 nm for methylene blue and safranine O, respectively. Results: In both the methods, the calibration curve was linear from 1–15 µg mL-1 of drug. Apparent molar absorpitivities were 1.7911 x 105, 2.3272 x 105 L mol-1 cm-1; Sandell’s sensitivities were 0.0215, 0.0162 µg cm-2; LOD were 0.182, 0.175 µg mL-1; LOQ were 0.551, 0.531 µg mL-1 for methods MB and SO, respectively. The relative standard deviation and percent recovery ranged from 0.206–1.310% and 99.0–101.5%, respectively. Conclusion: The results demonstrate that the proposed methods are sensitive, precise, accurate and inexpensive. These methods can easily be used for the assay of ABS in quality control laboratories.





Comments
First name  
Last name  
Email address  
Comments  
Security code



This Article
PDF

Google Scholar
Articles by Kumar NS
Articles by Rani AP
Articles by Visalakshi T
Articles by Sekharan CB

PubMed
Articles by Kumar NS
Articles by Rani AP
Articles by Visalakshi T
Articles by Sekharan CB

Similar articles in PubMed

Share this article!

Press Manuscript Online. Powered by MAADRAYAN