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Advanced Pharmaceutical Bulletin
ISSN: 2228-5881      eISSN: 2251-7308  
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Article History
Submitted: 30 Oct 2012
Revised: 23 Nov 2012
First published online: 07 Feb 2013

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Adv Pharm Bull. 2013;3(1):183-188 doi: 10.5681/apb.2013.030
PMID:24312833        PMCID:PMC3846065

High Performance Liquid Chromatographic Analysis of Almotriptan Malate in Bulk and Tablets

Original Research

Petikam Lavudu, Avula Prameela Rani, Chepuri Divya, Chandra Bala Sekharan *


Abstract
Purpose: A simple RP-HPLC method has been developed and validated for the determination of almotriptan malate (ATM) in bulk and tablets. Methods: Chromatographic separation of ATM was achieved by using a Thermo Scientific C18 column. A Mobile phase containing a mixture of methanol, water and acetic acid (4:8:0.1 v/v) was pumped at the flow rate of 1 mL/min. Detection was performed at 227 nm. According to ICH guidelines, the method was validated. Results: The calibration curve was linear in the concentration range 5–60 µg/mL for the ATM with regression coefficient 0.9999. The method was precise with RSD <1.2%. Excellent recoveries of 99.60 - 100.80% proved the accuracy of the method. The limits of detection and quantification were found to be 0.025 and 0.075 µg/mL, respectively. Conclusion: The method was successfully applied for the quantification of ATM in tablets with acceptable accuracy and precision.





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