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Advanced Pharmaceutical Bulletin
ISSN: 2228-5881      eISSN: 2251-7308  
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Article History
Submitted: 25 Oct 2012
Revised: 26 Nov 2012
First published online: 07 Feb 2013

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Adv Pharm Bull. 2013;3(1):189-195 doi: 10.5681/apb.2013.031
PMID:24312834        PMCID:PMC3846035

Targeted Fluoromagnetic Nanoparticles for Imaging of Breast Cancer MCF-7 Cells

Original Research

Mostafa Heidari Majd, Jaleh Barar * , Davoud Asgari, Hadi Valizadeh, Mohammad Reza Rashidi, Vala Kafil, Javid Shahbazi, Yadollah Omidi


Abstract
Purpose: To achieve simultaneous imaging and therapy potentials, targeted fluoromagnetic nanoparticles were synthesized and examined in human breast cancer MCF-7 cells. Methods: Fe3O4 nanoparticles (NPs) were synthesized through thermal decomposition of Fe(acac)3. Then, magnetic nanoparticles (MNPs) modified by dopamine-poly ethylene glycol (PEG)-NH2; finally, half equivalent fluorescein isothiocyanate (FITC) and half equivalent folic acid were conjugated to one equivalent of it. The presence of Fe3O4-DPA-PEG-FA/FITC in the folate receptor (FR) positive MCF-7 cells was determined via fluorescent microscopy to monitor the cellular interaction of MNPs. Results: FT-IR spectra of final compound confirmed existence of fluorescein on folic acid grafted MNPs. The Fe3O4-DPA-PEG-FA/FITC NPs, which displayed a size rang about 30-35 nm using scanning electron microscopy (SEM) and transmission electron microscopy (TEM), were able to actively recognize the FR-positive MCF-7 cells, but not the FR-negative A549 cells. Conclusion: The uniform nano-sized Fe3O4-DPA-PEG-FA/FITC NPs displayed great potential as theranostics and can be used for targeted imaging of various tumors that overexpress FR.





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Articles by Heidari Majd M
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