Tabriz University of Medical Sciences About    Newsletter    Contact Us    Create Account    Log in  
Advanced Pharmaceutical Bulletin
ISSN: 2228-5881      eISSN: 2251-7308  
Services
Export citation
EndNote
Reference Manager
BibTeX
Medlars
Refworks
Mendeley

Cite by
Google Scholar
PMC(0)


Article History
Submitted: 09 Nov 2013
Revised: 28 Nov 2013
First published online: 07 Feb 2014

Article Access Statistics
Abstract Page Views: 383
PDF Downloads: 148
Full Text Views: 0

Adv Pharm Bull. 2014;4(3):267-272 doi: 10.5681/apb.2014.039
PMID:24754011        PMCID:PMC3992963

Chiral Separation of Indapamide Enantiomers by Capillary Electrophoresis

Original Research

Amelia Tero-Vescan 1, Gabriel Hancu 2 * , Mihaela Oroian 2, Anca Cârje 3

1 Department of Biochemistry, Faculty of Pharmacy, University of Medicine and Pharmacy, Târgu Mureş, Romania.
2 Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Medicine and Pharmacy, Târgu Mureş, Romania.
3 Department of Drug Analysis and Analytical Chemistry, Faculty of Pharmacy, University of Medicine and Pharmacy, Târgu Mureş, Romania.



Abstract
Purpose: Indapamide is probably the most frequently prescribed diuretic drug, generally being used for the treatment of hypertension. It contains a chiral center in its molecule; is marketed as a racemic mixture; but there are rather few studies regarding the pharmacokinetic and the pharmacological effect differences of the two enantiomers. Our aim was the development of a simple, rapid and precise analytical procedure for the chiral separation of indapamide enantiomers.Methods: In this study capillary zone electrophoresis was used for the enantiomeric separation of indapamide using a systematic screening approach involving different native and derivatized; neutral and charged cyclodextrines as chiral selectors. The effects of pH value and composition of the background electrolyte, capillary temperature, running voltage and injection parameters have been investigated.Results: After preliminary analysis a charged derivatized CD, sulfobuthyl ether- β-CD, proved to be the optimum chiral selector for the enantioseparation. Using a buffer solution containing 25 mM disodium hydrogenophosphate – 25 mM sodium didydrogenophosphate and 5 mM sulfobuthyl ether- β-CD as chiral selector at a pH - 7, a voltage of + 25 kV, temperature 15°C and UV detection at 242 nm, we succeeded in the separation of the two enantiomers in approximately 6 minutes, with a resolution of 4.30 and a separation factor of 1.08.Conclusion: Capillary zone electrophoresis using cyclodextrines as chiral selectors proved to be a suitable method for the enantioseparation of indapamide. Our method is rapid, specific, reliable, and cost-effective and can be proposed for laboratories performing indapamide routine analysis.





Comments
First name  
Last name  
Email address  
Comments  
Security code



This Article
PDF

Google Scholar
Articles by Tero-Vescan A
Articles by Hancu G
Articles by Oroian M
Articles by Cârje A

PubMed
Articles by Tero-Vescan A
Articles by Hancu G
Articles by Oroian M
Articles by Cârje A

Similar articles in PubMed

Share this article!

Press Manuscript Online. Powered by MAADRAYAN