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Advanced Pharmaceutical Bulletin
ISSN: 2228-5881      eISSN: 2251-7308  
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Article History
Submitted: 25 Aug 2013
Revised: 19 Sep 2013
First published online: 24 Dec 2013

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Adv Pharm Bull. 2014;4(2):161-165 doi: 10.5681/apb.2014.024
PMID:24511480        PMCID:PMC3915816

Simultaneous Determination of Loratadine, Desloratadine and Cetirizine by Capillary Zone Electrophoresis

Original Research

Gabriel Hancu 1 * , Camelia Campian 1, Aura Rusu 1, Eleonora Mircia 2, Hajnal Kelemen 1

1 Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Medicine and Pharmacy, Târgu Mureş, Romania.
2 Department of Organic Chemistry, Faculty of Pharmacy, University of Medicine and Pharmacy, Târgu Mureş, Romania.



Abstract
Purpose: The aim of the study was the development of a simple and rapid analytical procedure for the determination of the most frequently used antihistamine derivatives. Methods: A capillary zone electrophoretic method was developed for the simultaneous separation of loratadine, desloratadine and cetirizine. Efforts were focused primarly on the optimisation of the experimental parameters: buffer composition and concentration, buffer pH, applied voltage, temperature, injection pressure and time. Results: The optimised parameters for the separation were: 25 mM buffer electrolyte, buffer pH 2.5, voltage + 25 kV, temperature 25 °C, injection pressure 50 mbar, injection time 3 seconds, capillary 48 cm (effective length 40 cm) x 50 m, detection at 240 nm. Under these conditions, the analysis time was below 5 minutes, the order of migration being: desloratadine, cetirizine and loratadine. The developed method was validated in terms of linearity, limits of detection and quantification, intra- and inter-day precision, selectivity and robustness. Conclusion: Capillary zone electrophoresis proved to be a suitable method for the simulatneous determination of the three studied antihistamine derivatives.





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