Tabriz University of Medical Sciences About    Newsletter    Contact Us    Create Account    Log in  
Advanced Pharmaceutical Bulletin
ISSN: 2228-5881      eISSN: 2251-7308  
Services
Export citation
EndNote
Reference Manager
BibTeX
Medlars
Refworks
Mendeley

Cite by
Google Scholar



Article History
Submitted: 26 Jun 2016
Revised: 19 Nov 2016
Accepted: 24 Nov 2016
First published online: 22 Dec 2016

Article Access Statistics
Abstract Page Views: 102
PDF Downloads: 105
Full Text Views: 0

Adv Pharm Bull. 2016;6(4):651-654 doi: 10.15171/apb.2016.080

Purification and Characterization of Bovine Serum Albumin Using Chromatographic Method



Short Communication

Sanaz Balkani 1,2, Sara Shamekhi 1, Ramin Raoufinia 1, Reza Parvan 1, Jalal Abdolalizadeh 3,4 *

1 Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
2 University of Tehran Kish International Campus, Tehran University, Tehran, Iran.
3 Paramedical faculty, Tabriz University of Medical Sciences, Tabriz, Iran.
4 Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.



Abstract
Purpose: Albumin is an abundant protein of blood and has many biopharmaceutical applications. The aim of this study was to purify bovine serum albumin (BSA) using produced rabbit anti-BSA antibody. Methods: The polyclonal antibody was produced against the BSA in rabbits. Then, the pure BSA was injected to three white New Zealand rabbits. ELISA test was done to evaluate antibody production. After antibody purification,the purified antibody was attached to CNBr-activated sepharose and finally it was used for purification of albumin from bovine serum. Western blotting analysis was used for functional assessment of immunoaffinity purified BSA. Results: The titer of anti-bovine albumin determined by ELISA was obtained 1: 256000. The SDS-PAGE showed up to 98% purity of isolated BSA and western blotting confirmed the BSA functionality. Purified bovine serum albumin by affinity chromatography showed a single band with molecular weight of 66 KDa. Conclusion: Affinity chromatography using produced rabbit anti-BSA antibody would be an economical and safe method for purification of BSA.





Comments
First name  
Last name  
Email address  
Comments  
Security code



This Article
PDF

Google Scholar
Articles by Balkani S
Articles by Shamekhi S
Articles by Raoufinia R
Articles by Parvan R
Articles by Abdolalizadeh J

PubMed
Articles by Balkani S
Articles by Shamekhi S
Articles by Raoufinia R
Articles by Parvan R
Articles by Abdolalizadeh J



Share this article!

Press Manuscript Online. Powered by MAADRAYAN