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Article History
Submitted: 11 Mar 2016
Revised: 14 Mar 2016
Accepted: 15 Mar 2016
First published online: 29 Jun 2016

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Adv Pharm Bull. 2016;6(2):153-161 doi: 10.15171/apb.2016.022

Key Immune Cell Cytokines Affects the Telomere Activity of Cord Blood Cells In vitro

Research Article

Balal Brazvan 1,2, Raheleh Farahzadi 1, Seyede Momeneh Mohammadi 1,2, Soheila Montazer Saheb 1, Dariush Shanehbandi 3, Laurent Schmied 4, Jafar Soleimani Rad 1, Masoud Darabi 5, Hojjatollah Nozad Charoudeh 1 *

1 Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
2 Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran.
3 Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
4 Immunotherapy Laboratories, Department of Biomedicine, University Hospital Basel, Basel, Switzerland.
5 Department of Clinical Biochemistry and Laboratory Medicine, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.



Abstract

Purpose: Telomere is a nucleoprotein complex at the end of eukaryotic chromosomes and its length is regulated by telomerase. The number of DNA repeat sequence (TTAGGG)n is reduced with each cell division in differentiated cells. The aim of this study was to evaluate the effect of SCF (Stem Cell Factor), Flt3 (Fms- Like tyrosine kinase-3), Interleukin-2, 7 and 15 on telomere length and hTERT gene expression in mononuclear and umbilical cord blood stem cells (CD34+ cells) during development to lymphoid cells.

Methods: The mononuclear cells were isolated from umbilical cord blood by Ficoll-Paque density gradient. Then cells were cultured for 21 days in the presence of different cytokines. Telomere length and hTERT gene expression were evaluated in freshly isolated cells, 7, 14 and 21 days of culture by real-time PCR. The same condition had been done for CD34+ cells but telomere length and hTERT gene expression were measured at initial and day 21 of the experiment.

Results: Highest hTERT gene expression and maximum telomere length were measured at day14 of MNCs in the presence of IL-7 and IL-15. Also, there was a significant correlation between telomere length and telomerase gene expression in MNCs at 14 days in a combination of IL-7 and IL-15 (r = 0.998, p =0.04). In contrast, IL-2 showed no distinct effect on telomere length and hTERT gene expression in cells.

Conclusion: Taken together, IL-7 and IL-15 increased telomere length and hTERT gene expression at 14 day of the experiment. In conclusion, it seems likely that cells maintain naïve phenotype due to prolonged exposure of IL-7 and IL-15.







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Articles by Brazvan B
Articles by Farahzadi R
Articles by Mohammadi SM
Articles by Montazer Saheb S
Articles by Shanehbandi D
Articles by Schmied L
Articles by Soleimani Rad J
Articles by Darabi M
Articles by Nozad Charoudeh H

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Articles by Brazvan B
Articles by Farahzadi R
Articles by Mohammadi SM
Articles by Montazer Saheb S
Articles by Shanehbandi D
Articles by Schmied L
Articles by Soleimani Rad J
Articles by Darabi M
Articles by Nozad Charoudeh H



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