Tabriz University of Medical Sciences About    Newsletter    Contact Us    Create Account    Log in  
Advanced Pharmaceutical Bulletin
ISSN: 2228-5881      eISSN: 2251-7308  
Services
Export citation
EndNote
Reference Manager
BibTeX
Medlars
Refworks
Mendeley

Cite by
Google Scholar
PMC(0)


Article History
Submitted: 07 Oct 2012
Revised: 09 Oct 2012
First published online: 07 Feb 2013

Article Access Statistics
Abstract Page Views: 270
PDF Downloads: 125
Full Text Views: 0

Adv Pharm Bull. 2013;3(1):109-113 doi: 10.5681/apb.2013.018
PMID:24312821        PMCID:PMC3846050

Mass-Production and Characterization of Anti-CD20 Monoclonal Antibody in Peritoneum of Balb/c Mice

Original Research

Koushan Sineh sepehr, Behzad Baradaran * , Jafar Majidi, Jalal Abdolalizadeh, leili Aghebati, Fatemeh Zare Shahneh


Abstract
Purpose: Monoclonal antibodies are important tools are used in basic research as well as, in diagnosis, imaging and treatment of immunodeficiency diseases, infections and cancers. The purpose of this study was to produce large scale of monoclonal antibody against CD20 in order to diagnostic application in leukemia and lymphomas disorders. Methods: Hybridoma cells that produce monoclonal antibody against human CD20 were administered into the peritoneum of the Balb/c mice which have previously been primed with 0.5 ml Pristane. After twelve days, approximately 7 ml ascetic fluid was harvested from the peritoneum of each mouse. Evaluation of mAb titration was assessed by ELISA method. In the present study, we describe a protocol for large scale production of MAbs. Results: We prepared monoclonal antibodies (mAbs) with high specificity and sensitivity against human CD20 by hybridoma method and characterized them by ELISA. The subclass of antibody was IgG2a and its light chain was kappa. Ascetic fluid was purified by Protein-A Sepharose affinity chromatography and the purified monoclonal antibody was conjugated with FITC and Immunofluorescence was done for confirming the specific binding. Conclusion: The conjugated monoclonal antibody could have application in diagnosis B-cell lymphomas, hairy cell leukemia, B-cell chronic lymphocytic leukemia, and melanoma cancer stem cells.





Comments
First name  
Last name  
Email address  
Comments  
Security code



This Article
PDF

Google Scholar
Articles by Sineh sepehr K
Articles by Baradaran B
Articles by Majidi J
Articles by Abdolalizadeh J
Articles by Aghebati L
Articles by Zare Shahneh F

PubMed
Articles by Sineh sepehr K
Articles by Baradaran B
Articles by Majidi J
Articles by Abdolalizadeh J
Articles by Aghebati L
Articles by Zare Shahneh F

Similar articles in PubMed

Share this article!

Press Manuscript Online. Powered by MAADRAYAN