Abstract
Purpose: Citrus is
considered as a medically important plant from ancient times and the
bioflavonoids of different variety of citrus fruits were well explored for
their biological activities. The study aim
was to explore the effect of citrus bioflavonoids (CB) to prevent and cure
hyperoxaluria induced urolithiasis.
Methods: Twenty four Wistar rats were segregated into 4 Groups.
Group 1: Control; Group 2: Urolithic (EG-0.75%); Group 3: Preventive study
(EG+CB, day 1-50); Group 4: Curative study (EG+CB, day 30-50). Animals received
CB orally (20mg/kg body weight) after performing a toxicity study.
Results: Urinary risk factors and serum renal function
parameters were significantly reduced by CB administration in both preventive
and curative study (p<0.001). Hematoxylin & Eosin and von Kossa staining
demonstrated that renal protection was offered by CB against EG insult.
Immunohistochemical analyses revealed over expression and abnormal localization
of THP and NF-KB in urolithic rats, while it was effectively regulated by CB
supplementation.
Conclusions: CB prevented and significantly controlled lithogenic
factors and CaOx deposition in rats. We propose CB as a potential therapy in
management of urolithiasis.