Purpose: Mammalian target of rapamycin (mTOR)is important in hematopoiesis and
affect cell growth,differentiation and survival.
Although previous studies were identified the effect of cytokines on the
mononuclear cells development however the cytokines effect on mTOR in cord
blood mononuclear cells was unclear. The aim of this study was to evaluate mTOR expression in cord blood mononuclear
and cord blood stem cells (CD34+ cells) in culture conditions for
lymphoid cell development.
Methods: Isolation of The mononuclear cells (MNCs) from umbilical cord blood were
done with use of Ficollpaque density gradient. We evaluated cultured cord blood
mononuclear and CD34+ cells in presece of IL2, IL7 and IL15 at
distinct time points during 21 days by using flow cytometry. In this study, we
presented the role of IL2, IL7 and IL15 on the expression of mTOR in cord blood
Results: mTOR expression were increased in peresence of IL2, IL7 and IL15 in day
14 and afterword reduced. However in persence of IL2 and IL15 expression of
mTOR significantly reduced. mTOR expression in CD34+ cells
decreased significantly from day7 to day 21 in culture.
Conclusion: cytokines play important
role in mTOR expression during hematopoiesis and development of cord blood