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Advanced Pharmaceutical Bulletin
ISSN: 2228-5881      eISSN: 2251-7308  
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Article History
Submitted: 15 Aug 2016
Revised: 22 Jan 2017
Accepted: 24 Jan 2017
First published online: 13 Apr 2017

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Adv Pharm Bull. 2017;7(1):81-85 doi: 10.15171/apb.2017.010

Cord Blood Cells Responses to IL2, IL7 and IL15 Cytokines for mTOR Expression

Research Article

Anahita Mohammadian 1, Elahe Naderali 1, Seyedeh Momeneh Mohammadi 1, Aliakbar Movasaghpour 1, Behnaz Valipour 1, Mohammad Nouri 1, Hojjatollah Nozad Charoudeh 1 *

1 Stem Cell Research Center, Tabriz university of Medical Sciences, Tabriz, Iran.



Abstract

Purpose: Mammalian target of rapamycin (mTOR)is important in hematopoiesis and affect cell growth,differentiation and survival. Although previous studies were identified the effect of cytokines on the mononuclear cells development however the cytokines effect on mTOR in cord blood mononuclear cells was unclear. The aim of this study was to evaluate mTOR expression in cord blood mononuclear and cord blood stem cells (CD34+ cells) in culture conditions for lymphoid cell development.

Methods: Isolation of The mononuclear cells (MNCs) from umbilical cord blood were done with use of Ficollpaque density gradient. We evaluated cultured cord blood mononuclear and CD34+ cells in presece of IL2, IL7 and IL15 at distinct time points during 21 days by using flow cytometry. In this study, we presented the role of IL2, IL7 and IL15 on the expression of mTOR in cord blood cells.

Results: mTOR expression were increased in peresence of IL2, IL7 and IL15 in day 14 and afterword reduced. However in persence of IL2 and IL15 expression of mTOR significantly reduced. mTOR expression in CD34+ cells decreased significantly from day7 to day 21 in culture.

Conclusion: cytokines play important role in mTOR expression during hematopoiesis and development of cord blood mononuclear cells.



Key words
Cord blood, mTOR, Cytokines





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Articles by Mohammadian A
Articles by Naderali E
Articles by Mohammadi SM
Articles by Movasaghpour A
Articles by Valipour B
Articles by Nouri M
Articles by Nozad Charoudeh H

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Articles by Mohammadian A
Articles by Naderali E
Articles by Mohammadi SM
Articles by Movasaghpour A
Articles by Valipour B
Articles by Nouri M
Articles by Nozad Charoudeh H



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