Nahid Khaziri
1, Momeneh Mohammadi
1, Zeinab Aliyari
1, Jafar Soleimani Rad
2, Hamid Tayefi Nasrabadi
1,2, Hojjatollah Nozad Charoudeh
1,2*1 Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
2 Tissue Engineering Group, Novin School of Advanced Research Sciences, Tabriz University of Medical Sciences, Tabriz, Iran.
Abstract
Purpose: Although bone marrow represents the main site for NK cell
development and also distinct thymic-dependentNK cell pathway was identified,
the cytokines effect on the NK cell generation from cord blood is unclear.
Studies were identified the role of cytokines in the regulation of bone marrow
and thymic NK cells. Previous studies reported that IL15 are critical for bone marrow dependent
and IL7 is important for thymic NK cells. It is remain unclear the cytokines
influence on the expantion of NK cells in cord blood mononuclear cells.
Methods: We evaluated cultured cord blood mononuclear cells
suplememnted with combinations of cytokines
using FACS in distinct time points.
In this study, we presented the role of IL2, IL7 and IL15 as members of
the common gamma receptor -chain
(Il2rg) on the expansion NK cells from
cord blood cells.
Results: By investigating cord blood mononuclear cells in vitro , we
demonstrated that IL2 and IL15 are important for expansion of NK cells. IL2 in
comparision with IL15 has more influences in NK cell expansion. In contrast
IL-7 is dispensable for NK cell generation in cord blood.
Conclusion: Thus,IL-2Rg cytokines play
complementary roles and are indispensable for homeostasis of NK cell
development in cord blood. Probably these cytokines could help to use NK
beneficials in engrafment of transplanted cells and Anti tumor activity of NK cells.