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Article History
Submitted: 06 Jul 2014
Revised: 14 Sep 2014
First published online: 01 Jun 2015

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Adv Pharm Bull. 2015;5(2):261-267 doi: 10.15171/apb.2015.036
PMID:26236666        PMCID:PMC4517085

Increased P-35, EBI3 Transcripts and Other Treg Markers in Peripheral Blood Mononuclear Cells of Breast Cancer Patients with Different clinical Stages

Original Research

Maryam Hamidinia 1,2, Mehri Ghafourian Boroujerdnia 1, Abdolhassan Talaiezadeh 2,3, Ghasem Solgi 4, Reyhaneh Roshani 1,2, Sara Iranprast 1,2, Ali Khodadadi 1,2 *

1 Department of Immunology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
2 Cancer Petroleum and Environmental Pollutants Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
3 Department of Surgery, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
4 Department of Immunology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.



Abstract
Purpose: Currently, cancer as a major problem around the world threatens human health and has a high incidence in developing countries. Many reports have indicated that patients suffering from cancer demonstrate decreased antitumor immune responses as well as a high prevalence of T regulatory population. It has been reported that Foxp3+Tregs exert suppression by cell contact-dependent mechanisms which are mediated by soluble factors such as immunosuppressive cytokines like IL-10, TGF-​B and IL-35. Consequently there is a great need to identify prognostic and diagnostic biomarkers of regulatory T cells for vaccine and drug development. Methods: In this study IL-10, TGF-B, IL-35 and Foxp3 mRNA gene expression has been measured in peripheral blood of 40 breast cancer patients and 40 normal age-matched women using quantitative real-time PCR (qRT-PCR) method with Master Mix reaction containing SYBER Green. GAPDH gene was used as housekeeping gene. Results: Our data demonstrated a significant up-regulation of IL-10, TGF-​B, P35, EBI3 and Foxp3 gene expression in patients’ peripheral blood compared to normal healthy controls (p<0.05). Conclusion: The data suggests that the immune system is suppressed in breast cancer patients, which may be due to elevated Treg cells population. These results may be useful for diagnostic or therapeutic purposes. However it may require more investigations





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Articles by Hamidinia M
Articles by Ghafourian Boroujerdnia M
Articles by Talaiezadeh A
Articles by Solgi G
Articles by Roshani R
Articles by Iranprast S
Articles by Khodadadi A

PubMed
Articles by Hamidinia M
Articles by Ghafourian Boroujerdnia M
Articles by Talaiezadeh A
Articles by Solgi G
Articles by Roshani R
Articles by Iranprast S
Articles by Khodadadi A

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