Logo-apb
Adv Pharm Bull. 2017;7(2): 241-249.
doi: 10.15171/apb.2017.029
PMID: 28761826
PMCID: PMC5527238
Scopus ID: 85021428280
  Abstract View: 3304
  PDF Download: 1681

Research Article

The Inhibitory Effect of Ginger Extract on Ovarian Cancer Cell Line; Application of Systems Biology

Roghiyeh Pashaei-Asl 1,2, Fatima Pashaei-Asl 3, Parvin Mostafa Gharabaghi 4*, Khodadad Khodadadi 5, Mansour Ebrahimi 6, Esmaeil Ebrahimie 7,8,9,10, Maryam Pashaiasl 4,11,12*

1 Department of Anatomy, Medical School, Iran University of Medical Science, Tehran, Iran.
2 Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran.
3 Molecular Biology Laboratory, Biotechnology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
4 Women’s Reproductive Health Research Center, Tabriz University of Medical Sciences Tabriz, Iran.
5 Genetic Research Theme, Murdoch Children’s Research Institute, Royal Children's Hospital, The University of Melbourne, Melbourne, Australia.
6 Department of Biology, University of Qom, Qom, Iran.
7 Institute of Biotechnology, Shiraz University, Shiraz, Iran.
8 School of Biological Sciences, Faculty of Science and Engineering, Flinders University, Adelaide, Australia.
9 School of Information Technology and Mathematical Sciences, Division of Information Technology, Engineering and the Environment, The University of South Australia, Adelaide, Australia.
10 School of Animal & Veterinary Science, The University of Adelaide, Australia.
11 Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
12 Department of Anatomical Sciences, Faculty of Medicine, Tabriz University of Medical Sciences, Iran.
*Corresponding Author: Department of Anatomical Sciences, Faculty of Medicine, Tabriz University of Medical Sciences, Iran. Email pm_gharabaghi@yahoo.com, pashaim@tbzmed.ac.ir
*Corresponding Authors: Email: pm_gharabaghi@yahoo.com; Email: pashaim@tbzmed.ac.ir

Abstract

Purpose: Ginger is a natural compound with anti-cancer properties. The effects of ginger and its mechanism on ovarian cancer and its cell line model, SKOV-3, are unclear. In this study, we have evaluated the effect of ginger extract on SKOV-3.

Methods: SKOV-3 cells were incubated with ginger extract for 24, 48 and 72 hours. Cell toxicity assay was performed. Different data mining algorithms were applied to highlight the most important features contributing to ginger inhibition on the SKOV-3 cell proliferation. Moreover, Real-Time PCR was performed to assay p53, p21 and bcl-2 genes expression. For co-expression meta-analysis of p53, mutual ranking (MR) index and transformation to Z-values (Z distribution) were applied on available transcriptome data in NCBI GEO data repository.

Results: The ginger extract significantly inhibited cancer growth in ovarian cancer cell line. The most important attribute was 60 µg/ml concentration which received weights higher than 0.50, 0.75 and 0.95 by 90%, 80% and 50% of feature selection models, respectively. The expression level of p53 was increased sharply in response to ginger treatment. Systems biology analysis and meta-analysis of deposited expression value in NCBI based on rank of correlation and Z-transformation approach unraveled the key co-expressed genes and co-expressed network of P53, as the key transcription factor induced by ginger extract. High co-expression between P53 and the other apoptosis-inducing proteins such as CASP2 and DEDD was noticeable, suggesting the molecular mechanism underpinning of ginger action.

Conclusion: We found that the ginger extract has anticancer properties through p53 pathway to induce apoptosis.


First Name
 
Last Name
 
Email Address
 
Comments
 
Security code


Abstract View: 3268

Your browser does not support the canvas element.


PDF Download: 1681

Your browser does not support the canvas element.

Submitted: 23 Feb 2017
Revision: 09 May 2017
Accepted: 11 May 2017
ePublished: 30 Jun 2017
EndNote EndNote

(Enw Format - Win & Mac)

BibTeX BibTeX

(Bib Format - Win & Mac)

Bookends Bookends

(Ris Format - Mac only)

EasyBib EasyBib

(Ris Format - Win & Mac)

Medlars Medlars

(Txt Format - Win & Mac)

Mendeley Web Mendeley Web
Mendeley Mendeley

(Ris Format - Win & Mac)

Papers Papers

(Ris Format - Win & Mac)

ProCite ProCite

(Ris Format - Win & Mac)

Reference Manager Reference Manager

(Ris Format - Win only)

Refworks Refworks

(Refworks Format - Win & Mac)

Zotero Zotero

(Ris Format - Firefox Plugin)