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Advanced Pharmaceutical Bulletin
ISSN: 2228-5881      eISSN: 2251-7308  
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Article History
Submitted: 11 Sep 2015
Revised: 11 May 2016
Accepted: 14 May 2016
First published online: 29 Jun 2016

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Adv Pharm Bull. 2016;6(2):267-270 doi: 10.15171/apb.2016.037

Fisetin Protects DNA Against Oxidative Damage and Its Possible Mechanism

Short Communication

Tingting Wang, Huajuan Lin, Qian Tu, Jingjing Liu, Xican Li *

1 School of Chinese Herbal Medicine, Guangzhou University of Chinese Medicine, Waihuang East Road No.232, Guangzhou Higher Education Mega Center, 510006, Guangzhou, China.



Abstract
Purpose: The paper tries to assess the protective effect of fisetin against •OH-induced DNAdamage, then to investigate the possible mechanism.Methods: The protective effect was evaluated based on the content of malondialdehyde(MDA). The possible mechanism was analyzed using various antioxidant methods in vitro,including •OH scavenging (deoxyribose degradation), •O2- scavenging (pyrogallolautoxidation), DPPH• scavenging, ABTS•+ scavenging, and Cu2+-reducing power assays.Results: Fisetin increased dose-dependently its protective percentages against •OH-inducedDNA damage (IC50 value =1535.00±29.60 μM). It also increased its radical-scavengingpercentages in a dose-dependent manner in various antioxidants assays. Its IC50 values in•OH scavenging, •O2- scavenging, DPPH• scavenging, ABTS•+ scavenging, and Cu2+-reducing power assays, were 47.41±4.50 μM, 34.05±0.87 μM, 9.69±0.53 μM, 2.43±0.14μM, and 1.49±0.16 μM, respectively.Conclusion: Fisetin can effectively protect DNA against •OH-induced oxidative damagepossibly via reactive oxygen species (ROS) scavenging approach, which is assumed to behydrogen atom (H•) and/or single electron (e) donation (HAT/SET) pathways. In the HATpathway, the 3’,4’-dihydroxyl moiety in B ring of fisetin is thought to play an importantrole, because it can be ultimately oxidized to a stable ortho-benzoquinone form.





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