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Advanced Pharmaceutical Bulletin
ISSN: 2228-5881      eISSN: 2251-7308  
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Article History
Submitted: 03 Aug 2013
Revised: 12 Sep 2013
First published online: 24 Dec 2013

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Adv Pharm Bull. 2014;4(2):147-153 doi: 10.5681/apb.2014.022
PMID:24511478        PMCID:PMC3915814

DNA Protective Effect of Mangosteen Xanthones: an in Vitro Study on Possible Mechanisms

Original Research

Jing Lin 1, Yaoxiang Gao 1, Haiming Li 1, Lulu Zhang 1, Xican Li 1 *

1 School of Chinese Herbal Medicine, Guangzhou University of Chinese Medicine, Guangzhou, 510006, China.



Abstract
Purpose: The aim of this study was to evaluate antioxidant ability of mangosteen shell and explore the non-enzymatic repair reaction and possible mechanism of xanthones in mangosteen shell. Methods: Mangosteen shell was extracted by methanol to obtain the extract of mangosteen shell. The extract was then determined by various antioxidant assays in vitro, including protection against DNA damage, •OH scavenging,DPPH• (1,1-diphenyl-2-picryl-hydrazl radical) scavenging, ABTS+• (2,2′-azino-bis(3-ethylbenzo- thiazoline-6-sulfonic acid diammonium) scavenging, Cu2+-chelating, Fe2+-chelatingand Fe3+ reducing assays. Results: Mangosteen shell extract increased dose-dependently its percentages in all assays. Its IC50 values were calculated as 727.85±2.21,176.94±19.25, 453.91±6.47, 84.60±2.47, 6.81±0.28, 1.55±0.10, 3.93±0.17, and 9.52±0.53μg/mL, respectively for DNA damage assay, •OH scavenging assay, Fe2+-Chelating assay, Cu2+-Chelating assay, DPPH• scavenging assay, ABTS+•scavenging assay, Fe3+ reducing assay and Cu2+ reducing assay. Conclusion: On the mechanistic analysis, it can be concluded that mangosteen shell can effectively protect against hydroxyl-induced DNA oxidative damage. The protective effect can be attributed to the xanthones. One approach for xanthones to protect against hydroxyl-induced DNA oxidative damage may be ROS scavenging. ROS scavenging may be mediated via metal-chelating, and direct radical-scavenging which is through donating hydrogen atom (H·) and electron (e). However, both donating hydrogen atom (H·) and electron (e) can result in the oxidation of xanthone to stable quinone form.





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