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Advanced Pharmaceutical Bulletin
ISSN: 2228-5881      eISSN: 2251-7308  
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Article History
Submitted: 13 Dec 2016
Revised: 11 Mar 2017
Accepted: 14 Mar 2017
First published online: 13 Apr 2017

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Adv Pharm Bull. 2017;7(1):131-139 doi: 10.15171/apb.2017.017

Preparation, Optimization and Activity Evaluation of PLGA/Streptokinase Nanoparticles Using Electrospray

Research Article

Nasrin Yaghoobi 1, Reza Faridi Majidi 1, Mohammad ali Faramarzi 2, Hadi Baharifar 1, Amir Amani 1,3 *

1 Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.
2 Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
3 Medical Biomaterials Research Center (MBRC), Tehran University of Medical Sciences, Tehran, Iran.



Abstract

Purpose: PLGA nanoparticles (NPs) have been extensively investigated as carriers of different drug molecules to enhance their therapeutic effects or preserve them from the aqueous environment. Streptokinase (SK) is an important medicine for thrombotic diseases.

Methods: In this study, we used electrospray to encapsulate SK in PLGA NPs and evaluate its activity. This is the first paper which investigates activity of an electrosprayed enzyme. Effect of three input parameters, namely, voltage, internal diameter of needle (nozzle) and concentration ratio of polymer to protein on size and size distribution (SD) of NPs was evaluated using artificial neural networks (ANNs). Optimizing the SD has been rarely reported so far in electrospray.

Results: From the results, to obtain lowest size of nanoparticles, ratio of polymer/enzyme and needle internal diameter (ID) should be low. Also, minimum SD was obtainable at high values of voltage. The optimum preparation had mean (SD) size, encapsulation efficiency and loading capacity of 37 (12) nm, 90% and 8.2%, respectively. Nearly, 20% of SK was released in the first 30 minutes, followed by cumulative release of 41% during 72 h. Activity of the enzyme was also checked 30 min after preparation and 19.2% activity was shown.

Conclusion: Our study showed that electrospraying could be an interesting approach to encapsulate proteins/enzymes in polymeric nanoparticles. However, further works are required to assure maintaining the activity of the enzyme/protein after electrospray.




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Articles by Yaghoobi N
Articles by Faridi Majidi R
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Articles by Yaghoobi N
Articles by Faridi Majidi R
Articles by Faramarzi MA
Articles by Baharifar H
Articles by Amani A



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