Tabriz University of Medical Sciences About    Newsletter    Contact Us    Create Account    Log in  
Advanced Pharmaceutical Bulletin
ISSN: 2228-5881      eISSN: 2251-7308  
Services
Export citation
EndNote
Reference Manager
BibTeX
Medlars
Refworks
Mendeley

Cite by
Google Scholar
PMC(1)


Article History
Submitted: 27 Jan 2014
Revised: 02 Mar 2014
First published online: 25 Aug 2014

Article Access Statistics
Abstract Page Views: 352
PDF Downloads: 146
Full Text Views: 0

Adv Pharm Bull. 2014;4(5):437-441 doi: 10.5681/apb.2014.064
PMID:25364659        PMCID:PMC4213782

Glycyrrhetinic Acid Inhibits Cell Growth and Induces Apoptosis in Ovarian Cancer A2780 Cells

Original Research

Venus Haghshenas, Shohreh Fakhari, Sako Mirzaie, Mohammadreza Rahmani, Fariba Farhadifar, Sara Peirzadeh, Ali Jalili *

1 Department of Biochemistry, Research and Science, Islamic Azad University, Sanandaj, Iran.
2 Kurdistan Cellular and Molecular Research Center, Kurdistan University of Medical Sciences, Sanandaj, Iran.
3 Department of Biochemistry, Sanandaj Branch, Islamic Azad University, Iran.
4 Department of Gynecology, Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran.



Abstract
Purpose: Accumulating evidence indicates that glycyrrhizin (GZ) and its hydrolyzed metabolite 18-β glycyrrhetinic acid (GA) exhibit anti-inflammatory and anticancer activities. The objective of this study was to examine the in vitro cytotoxic activity of GA on human ovarian cancer A2780 cells. Methods: A2780 cells were cultured in RPMI1640 containing 10% fetal bovine serum. Cells were treated with different doses of GA and cell viability and proliferation were detected by dye exclusion and 3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) assays. Apoptosis induction and expression of Fas and Fas ligand (FasL) were analyzed by flow cytometry. Results: We observed that GA decreases cell viability and suppressed cells proliferation in a dose-dependent manner as detected by dye-exclusion and XTT assayes. In addition, our flow cytometry data show that GA not only induces apoptosis in A2780 cells but also upregulates both Fas and FasL on these cells in a dose-dependent manner. Conclusion: we demonstrate that GA causes cell death in A2780 cells by inducing apoptosis.





Comments
First name  
Last name  
Email address  
Comments  
Security code



This Article
PDF

Google Scholar
Articles by Haghshenas V
Articles by Fakhari S
Articles by Mirzaie S
Articles by Rahmani M
Articles by Farhadifar F
Articles by Peirzadeh S
Articles by Jalili A

PubMed
Articles by Haghshenas V
Articles by Fakhari S
Articles by Mirzaie S
Articles by Rahmani M
Articles by Farhadifar F
Articles by Peirzadeh S
Articles by Jalili A

Similar articles in PubMed

Share this article!

Press Manuscript Online. Powered by MAADRAYAN