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Advanced Pharmaceutical Bulletin
ISSN: 2228-5881      eISSN: 2251-7308  
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Article History
Submitted: 04 Jan 2014
Revised: 07 Feb 2014
First published online: 25 Aug 2014

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Adv Pharm Bull. 2014;4(5):429-436 doi: 10.5681/apb.2014.063
PMID:25364658        PMCID:PMC4213781

Antitumor Activity of Kielmeyera Coriacea Leaf Constituents in Experimental Melanoma, Tested in Vitro and in Vivo in Syngeneic Mice

Original Research

Carlos Rogerio Figueiredo 1 * , Alisson Leonardo Matsuo 1, Mariana Hiromi Massaoka 1, Natalia Girola 1, Ricardo Alexandre Azevedo 1, Aline Nogueira Rabaça 1, Camyla Fernandes Farias 1, Felipe Valença Pereira 1, Natalia Silva Matias 2, Luciana Pereira Silva 2, Elaine Guadelupe Rodrigues 1, Joao Henrique Guilardi Lago 3, Luiz Rodolpho Travassos 1, Regildo Márcio Gonçalves Silva 2

1 Department of Microbiology, Immunology and Parasitology, Experimental Oncology Unit (UNONEX), Federal University of São Paulo (UNIFESP), São Paulo, Brazil.
2 Department of Biological Sciences – Laboratory of Herbal Medicines, Universidade Estadual Paulista (UNESP- FLC/Assis), São Paulo, Brazil.
3 Institute of Environmental, Chemical and Pharmaceutical Sciences, Federal University of São Paulo (UNIFESP), Diadema, São Paulo, SP, Brazil.



Abstract
Purpose: The antitumor activity of Kielmeyera coriacea (Clusiaceae), a medicinal plant used in the treatment of parasitic, as well as fungal and bacterial infections by the Brazilian Cerrado population, was investigated. Methods: A chloroform extract (CE) of K. coriacea was tested in the murine melanoma cell line (B16F10-Nex2) and a panel of human tumor cell lines. Tumor cell migration was determined by the wound-healing assay and the in vivo antitumor activity of CE was investigated in a melanoma cell metastatic model. 1H NMR and GC/MS were used to determine CE chemical composition. Results: We found that CE exhibited strong cytotoxic activity against murine melanoma cells and a panel of human tumor cell lines in vitro. CE also inhibited growth of B16F10-Nex2 cells at sub lethal concentrations, inducing cell cycle arrest at S phase, and inhibition of tumor cell migration. Most importantly, administration of CE significantly reduced the number of melanoma metastatic nodules in vivo. Chemical analysis of CE indicated the presence of the long chain fatty compounds, 1-eicosanol, 1-docosanol, and 2-nonadecanone as main constituents. Conclusion: These results indicate that K. coriacea is a promising medicinal plant in cancer therapy exhibiting antitumor activity both in vitro and in vivo against different tumor cell lines.





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