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Advanced Pharmaceutical Bulletin
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Article History
Submitted: 30 May 2016
Revised: 05 Jun 2016
Accepted: 06 Jun 2016
First published online: 30 Jun 2016

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Adv Pharm Bull. 2016;6(2):275-283 doi: 10.15171/apb.2016.039

Anti-Inflammatory and Antioxidant Activity of Acalypha hispida Leaf and Analysis of its Major Bioactive Polyphenols by HPLC

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Md. Afjalus Siraj 1 * , Jamil A. Shilpi 2, Md. Golam Hossain 2, Shaikh Jamal Uddin 2 * , Md. Khirul Islam 3, Ismet Ara Jahan 4, Hemayet Hossain 4

1 Department of Pharmaceutical Science, Daniel K. Inouye College of Pharmacy, University of Hawaii at Hilo, Hilo, HI 96720, USA.
2 Faculty of Pharmacy Discipline, Life Science School, Khulna University, Khulna 9208, Bangladesh.
3 Department of Biochemistry, Faculty of Mathematics and Natural Sciences, University of Turku, FI-20500, Finland.
4 BCSIR Laboratories, Bangladesh Council of Scientific and Industrial Research (BCSIR), Dhaka 1205, Bangladesh.


Purpose: Inflammation and oxidative stress can lead to different chronic diseases including cancer and atherosclerosis. Many medicinal plants have the potential to show as anti-inflammatory activity. Present investigation was performed to investigate anti-inflammatory, antioxidant activity, and quantification of selected bioactive plant polyphenols of the ethanol (EAH) and aqueous (AAH) extracts of Acalypha hispida (Euphorbiaceae) leaves.

Methods: Anti-inflammatory activity was evaluated by carragenan and histamine induced rat paw edema models while antioxidant capacity was evaluated by DPPH free radical scavenging, Fe+2 chelating ability, reducing power, NO scavenging, total phenolic and total flavonoid content assay. Identification and quantification of bioactive polyphenols was done by HPLC.

Results: At the doses of 200 and 400 mg/kg, both EAH and AAH showed statistically significant inhibition of paw volume in the anti-inflammatory activity test. Both the extracts showed DPPH scavenging (IC50: 14 and 17 µg/ml, respectively), Fe+2 ion chelating (IC50: 40 and 46 µg/ml, respectively), NO scavenging activity (65.49 and 60.66% inhibition at 100 µg/ml), and concentration dependent reducing power ability. For EAH and AAH, flavonoid content was 126.30 and 149.72 mg QE/g dry extract, while phenolic content was 130.51 and 173.80 mg GAE/g dry extract, respectively. HPLC analysis of EAH and AAH indicated the presence of high content of ellagic acid along with other phenolic constituents.

Conclusion: High content of ellagic acid along with other phenolic constituents might have played an important role in the observed anti-inflammatory and antioxidant activity.

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Articles by Siraj MA
Articles by Shilpi JA
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Articles by Siraj MA
Articles by Shilpi JA
Articles by Hossain MG
Articles by Uddin SJ
Articles by Islam MK
Articles by Jahan IA
Articles by Hossain H

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