Tabriz University of Medical Sciences About    Newsletter    Contact Us    Create Account    Log in  
Advanced Pharmaceutical Bulletin
ISSN: 2228-5881      eISSN: 2251-7308  
Services
Export citation
EndNote
Reference Manager
BibTeX
Medlars
Refworks
Mendeley

Cite by
Google Scholar
PMC(2)


Article History
Submitted: 13 Sep 2014
Revised: 07 Oct 2014
First published online: 05 Mar 2015

Article Access Statistics
Abstract Page Views: 366
PDF Downloads: 163
Full Text Views: 0

Adv Pharm Bull. 2015;5(1):41-49 doi: 10.5681/apb.2015.006
PMID:25789218        PMCID:PMC4352222

Synthesis and In Vitro Evaluation of Amphiphilic Peptides and Their Nanostructured Conjugates

Original Research

Samaneh Mohammadi 1, Javid Shahbazi Mojarrad 2, Parvin Zakeri-Milani 3, Ali Shirani 1, Samad Mussa Farkhani 1, Naser Samadi 1, Hadi Valizadeh 4 *

1 Faculty of Advanced Medical Sciences, Department of Medical Nanotechnology, Tabriz University of Medical Sciences, Tabriz, Iran.
2 Research Center for Pharmaceutical Nanotechnology and Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.
3 Liver and Gastrointestinal Diseases Research Center and Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.
4 Drug Applied Research Center and Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.



Abstract
Purpose: Breast cancer is the second leading cancer type among people of advanced countries. Various methods have been used for cancer treatment such as chemotherapy and radiotherapy. In the present study we have designed and synthesized a new group of drug delivery systems (DDS) containing a new class of Cell Penetrating Peptides (CPPs) named Peptide Amphiphiles (PAs). Methods: Two PAs and anionic peptides were synthesized using solid phase peptide synthesis (SPPS), namely [KW]4, [KW]5, E4 and E8. Then nano-peptides were synthesized by non-covalent binding between PAs and poly anions as [KW]4-E4, [KW]4-E8, [KW]5-E4 and [KW]5-E8. Results: Flow cytometry studies showed that increased chain length of PAs with a higher ratio between hydrophobicity and net charge results in increased intracellular uptake by MCF7 cells after 2h incubation. Moreover, nano-peptides showed greater intracellular uptake compared to PAs. Anti-proliferative assay revealed that by increasing chain length of PAs, the toxicity effect on MCF7 cells is reduced, however nano-peptides did not show significant toxicity on MCF7 cells even at high concentration levels. Conclusion: These data suggest that due to the lack of toxicity effect at high concentration levels and also high cellular uptake, nano-peptides are more suitable carrier compared to PAs for drug delivery.





Comments
First name  
Last name  
Email address  
Comments  
Security code



This Article
PDF

Google Scholar
Articles by Mohammadi S
Articles by Shahbazi Mojarrad J
Articles by Zakeri-Milani P
Articles by Shirani A
Articles by Mussa Farkhani S
Articles by Samadi N
Articles by Valizadeh H

PubMed
Articles by Mohammadi S
Articles by Shahbazi Mojarrad J
Articles by Zakeri-Milani P
Articles by Shirani A
Articles by Mussa Farkhani S
Articles by Samadi N
Articles by Valizadeh H

Similar articles in PubMed

Share this article!

Press Manuscript Online. Powered by MAADRAYAN